【生物土壤结皮和灌木根区土壤中细菌和真菌群落特征】Blaire Steven^a La Verne Gallegos-Graves^a Chris Yeager^a Jayne Belnap^b Cheryl R. Kuske^a . Common and distinguishing features of the bacterial and fungal communities in biological soil crusts and shrub root zone soils.Soil Biology and BiochemistryVolume 69 February 2014 Pages 302–312

Abstract

Soil microbial communities in dryland ecosystems play important roles as root associates of the widely spaced plants and as the dominant members of biological soil crusts (biocrusts) colonizing the plant interspaces. We employed rRNA gene sequencing (bacterial 16S-fungal large subunit) and shotgun metagenomic sequencing to compare the microbial communities inhabiting the root zones of the dominant shrub Larrea tridentata (creosote bush) and the interspace biocrusts in a Mojave desert shrubland within the Nevada Free Air CO₂ Enrichment (FACE) experiment. Most of the numerically abundant bacteria and fungi were present in both the biocrusts and root zones although the proportional abundance of those members differed significantly between habitats. Biocrust bacteria were predominantly Cyanobacteria while root zones harbored significantly more Actinobacteria and Proteobacteria. Pezizomycetes fungi dominated the biocrusts while Dothideomycetes were highest in root zones. Functional gene abundances in metagenome sequence datasets reflected the taxonomic differences noted in the 16S rRNA datasets