Nataša Šibanca b Alex J. Dumbrellc Ines Mandić-Mulecd Irena Mačeka b
. Impacts of naturally elevated soil CO2concentrations on communities of soil archaea and bacteria.Soil Biology and BiochemistryVolume 68 January 2014 Pages 348–356
Abstract
There is a limited understanding of the importance of abiotic factors in regulating biodiversity and structure of many functionally important soil microbial communities. In this paper we present a molecular characterisation of archaeal and bacterial communities exposed to long-term change in soil abiotic environment at natural CO2 springs (mofettes) using T-RFLP profiling and examination of 16S rRNA clone libraries. Our results show major shifts in archaeal and bacterial communities towards anaerobic and methanogenic taxa dominating in CO2 rich hypoxic soils with a significant increase in abundance of Methanomicrobia and predominantly anaerobic Chloroflexi and Firmicutes. O2 concentration in soil was consistently shown to be the strongest predictor of the compositional changes across both the archaeal and bacterial communities. However soil pH and total N were most important in separating the archaeal communities in transition and control zones but not the bacterial communitie