【中国不同农业土壤中氨氧化细菌的分布】Li-dong Shen^a Shuai Liu^a Li-ping Lou^a Wei-ping Liu^b Xiang-yang Xu^a Ping Zheng^a and Bao-lan Hu^a. Broad Distribution of Diverse Anaerobic Ammonium-Oxidizing Bacteria in Chinese Agricultural Soils. Appl. Environ. Microbiol. October 2013 vol. 79 no. 19 6167-6172

Abstract

Anaerobic ammonium-oxidizing (anammox) bacteria have been detected in many marine and freshwater ecosystems. However little is known about the distribution diversity and abundance of anammox bacteria in terrestrial ecosystems. In this study anammox bacteria were found to be present in various agricultural soils collected from 32 different locations in China. Phylogenetic analysis of the 16S rRNA genes showed “Candidatus Brocadia” “Candidatus Kuenenia” “Candidatus Anammoxoglobus” and “Candidatus Jettenia” in the collected soils with “Candidatus Brocadia” being the dominant genus. Quantitative PCR showed that the abundance of anammox bacteria ranged from 6.38 × 10⁴ ± 0.42 × 10⁴ to 3.69 × 10⁶ ± 0.25 × 10⁶ copies per gram of dry weight. Different levels of diversity composition and abundance of the anammox bacterial communities were observed and redundancy analysis indicated tha