【细胞分裂素：NO抑制物】Wei-Zhong Liu Dong-Dong Kong Xue-Xin Gu Hong-Bo Gao Jin-Zheng Wang Min Xia Qian Gao Li-Li Tian Zhang-Hong Xu Fang Bao Yong Hu Neng-Sheng Ye Zhen-Ming Pei and Yi-Kun He. Cytokinins can act as suppressors of nitric oxide inArabidopsis. PNAS 2013
Maintaining nitric oxide (NO) homeostasis is essential for normal plant physiological processes. However very little is known about the mechanisms of NO modulation in plants. Here we report a unique mechanism for the catabolism of NO based on the reaction with the plant hormone cytokinin. We screened for NO-insensitive mutants in Arabidopsis and isolated two allelic lines cnu1-1 and 1–2 (continuous NO-unstressed 1) that were identified as the previously reported altered meristem program 1 (amp1) and as having elevated levels of cytokinins. A double mutant of cnu1-2 and nitric oxide overexpression 1 (nox1) reduced the severity of the phenotypes ascribed to excess NO levels as did treating the nox1 line with trans-zeatin the predominant form of cytokinin in Arabidopsis. We further showed that peroxinitrite an active NO derivative can react with zeatin in vitro which together with the results