Li Cao Qiuxiang Yan Haiyan Ni Gang Hu Qing Hong and Shunpeng Li.Salinicola zeshuniisp. nov. a Moderately Halophilic Bacterium Isolated from Soil of a Chicken Farm. Current Microbiology 2012 DOI: 10.1007-s00284-012-0259-4.

Abstract

The taxonomic status of a moderately halophilic bacterium strain N4^T isolated from soil of a chicken farm in China was determined. It was Gram-negative nonspore-forming motile and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequence indicated that this strain belonged to the genus Salinicola as it showed the highest sequence similarities to Salinicola salaries M27^T (98.3 %) Salinicola socius SMB35T (98.1 %) and Salinicola halophilus CG4.1T (98.1 %). The major cellular fatty acids were C_16:0 (25.6 %) C_18:1ω7c (35.0 %) and C_19:0 cyclo x8c (11.9 %) which are properties shared by members of the genus Salinicola. The DNA G+C content of strain N4^T was 69.1 mol %. The level of DNA–DNA relatedness between strain N4^T and the other three type strains of the genus of Salinicola salaries M27T Salinicola socius SMB35T and Salinicola halophilus CG4.1T were 34.3 28.7 and 26.9 % respectively. Based on the results of phenotypic chemotaxonomic DNA–DNA relatedness and phylogenetic analysis strain N4T should be classified as a novel species of the genus Salinicola for which the name Salinicola zeshunii sp. nov. is proposed with strain N4T (=KACC 16602^T = CCTCC AB 2012912T) as the type strain.