【长江口潮间带沉积物中的氨氧化原核生物】Yanling Zhenga Lijun Houa Silvia Newellb Min Liuc Junliang Zhoua Hui Zhaod Lili Youa and Xunliang Chenga. Community Dynamics and Activity of Ammonia-Oxidizing Prokaryotes in Intertidal Sediments of the Yangtze Estuary. Appl. Environ. Microbiol. January 2014 vol. 80 no. 1 408-419
Abstract
Diversity abundance and activity of ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) were investigated using the ammonia monooxygenase α subunit (amoA) in the intertidal sediments of the Yangtze Estuary. Generally AOB had a lower diversity of amoA genes than did AOA in this study. Clone library analysis revealed great spatial variations in both AOB and AOA communities along the estuary. The UniFrac distance matrix showed that all the AOB communities and 6 out of 7 AOA communities in the Yangtze Estuary were statistically indistinguishable between summer and winter. The studied AOB and AOA community structures were observed to correlate with environmental parameters of which salinity pH ammonium total phosphorus and organic carbon had significant correlations with the composition and distribution of both communities. Also the AOA communities were significantly correlated with sediment clay content. Quantitative PCR (qPCR) results indicated that the abundance of AOB amoA genes was greater tha
